Acar Laboratory is located in the Gallogly Hall (opened in Fall 2019) at the University of Oklahoma, Norman campus. As a member of the Stephenson Cancer Center (NCI Designated), we have been closely working with collaborators at the University of Oklahoma, Health Sciences Center and Health Science Center (OUHSC), the Oklahoma Medical Research Foundation (OMRF).
The region has additional resources as in Oklahoma Bioscience Association (OKBio) and for translational research Oklahoma Innovation to Enterprise (i2E), and Oklahoma Center for the Advancement of Science & Technology (OCAST).
Synthesis and preparation:
Protein Technologies Prelude X peptide synthesizer with heated reaction vesicles and real-time UV monitoring
Branson 3800 Ultrasonic Cleaner
Sartorius Analytical and Precision Balances
Büchi Rotavap R-100
Kewaunee Scientific Corporation Fume Hoods
Mettler Toledo S220 pH Meter
Nova Automated Water Bath
PHCBI Minus 80°C Freezer
Purist Pro UV Ultrapure Water System
Steris AMSCO 250 LS Autoclaves
Keyence BZ-X800 Automated Fluorescence Microscope with optical sectioning
Agilent 1260 II HPLC system for purification of the synthesized peptides
Agilent Cary 5000 UV-Vis-NIR Spectrophotometer
Azur C600 UV-Vis-NIR Imaging System
BioTek Synergy Neo2 Multi-Mode Plate Reader
Horiba PTI Quanta Master 8000 Fluorometer
Olympus BX43F upright microscope for slide/histology imaging
Labconco Logic Plus Biological Safety Cabinet
Eppendorf Galaxy 170 S and CellXpert Cell Incubators - hypoxia assisted
DeNovix CellDrop BF Cell Counter and D11+ Spectrophotometer
Eppendorf 5427R and 5910R Centrifuges
Freezers (-20°C and -80°C) and Fridges (4°C)
Malvern ZetaSizer Nano ZS
MVE Cryosystem 6000 Cell Storage
Olympus CKX53 Optical Microscope
Olympus CKX53SF inverted microscope for general cell culture
Available core facilities:
Protein Production Core Facility: Located at OU’s Norman campus, this facility is created with the mission to aid researchers with customized and comprehensive solutions for protein expression and purification.
Chemistry and Biochemistry Core Facility: Located at OU’s Norman campus. Provides “Omics” data analysis (genomics, transcriptomics, metagenomics, comparative genomics), sequence analysis (assembly and SNP analysis), pathway analysis, customized analysis pipeline, experimental design assistance, access database constructions.
Mass Spectrometry & Proteomics: Located at OU’s Norman campus. The proteomics division of the Laboratory for Molecular Biology and Cytometry Research is committed to providing the highest quality services related to proteomic analysis, specifically biomolecule mass spectrometry. The instrumentation available for mass spectrometry services includes an ABSciex QSTAR Elite hybrid quadrupole/TOF mass spectrometer, a Bruker Daltonics – Ultraflex-2 MALDI-TOF, and a Waters Synapt G2-S High Definition mass spectrometers. Several of our mass spectrometers are coupled to HPLC instruments which allows us to provide additional applications to our researchers. The QStar is paired to 1D LC systems (Michrom Paradigm) while the Waters Synapt is paired to a 2D UPLC (nanoAcquity) in order to allow processing of more complex proteomic type samples.
DNA Sequencing/ Genomics: Located at OU’s HSC campus. The DNA Sequencing and Genomics facility offers an array of services aimed at facilitating the research of a variety of different investigators across a multitude of disciplines. Our current services include RNAseq, small genome sequencing, 16s metagenomics sequencing, ChIP seq, and small RNA seq using Illumina technology, small scale/custom DNA sequencing with or without plasmid prepping, primer extension and fragment analysis.
Samuel Roberts Noble Microscopy Laboratory: the core imaging facility of the University of Oklahoma houses the most sophisticated collection of electron and confocal microscopes in the State, with a combined capitalized value of nearly $4.5 million. Confocal microscopes are including Leica SP2 and Leica SP8 Multiphoton.
Flow Cytometry and Imaging: Located at the HSC campus. The Flow and Image Cytometry Laboratory provides the University of Oklahoma research community with state-of-the-art cell analysis and sorting instrumentation, and the technical expertise to best utilize this technology. The laboratory is equipped with a computer workstation and a variety of computer software packages to collect and analyze data. The facility maintains a sterile hood, CO2 incubator, centrifuge, and fluorescent microscope which can be used for cell preparation and tissue culture work.
Laboratory of Biomolecular Structure and Function: Located at OU’s HSC campus. The Laboratory of Biomolecular Structure and Function (LBSF) provides a local source of X-ray diffraction instruments with cryosystems that shorten the crystal optimization step. The LBSF also provides help with protein expression, purification, crystallization, data collection with synchrotron radiation, small angle X-ray scattering studies, molecular modeling, and structure-based drug design. In addition, the LBSF interfaces with the BMB Physical Biochemistry Facility for further biophysical characterization of pure protein (ligand binding and thermal stability studies).